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Development and bioreactor cultivation of a novel semidifferentiated tissue suspension derived from the marine plant Acrosiphonia coalita
Author(s) -
Rorrer Gregory L.,
Zhi Chunxing,
PolneFuller Miriam
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19960305)49:5<559::aid-bit9>3.0.co;2-i
Subject(s) - bioreactor , suspension culture , suspension (topology) , biochemical engineering , biology , microbiology and biotechnology , botany , engineering , mathematics , cell culture , homotopy , pure mathematics , genetics
A semidifferentiated tissue culture consisting of linear filaments in liquid suspension was established from Acrosiphonia coalita, a cold‐water green macroalga known to express pharmacologically active oxylipins deriving from lipoxygenase metabolism of linolenic acid. The tissue was vegatively propagated by blending the filaments down to 1 to 5 mm in length prior to subculture. The filamentous A. coalita tissue suspension was successfully cultivated in an illuminated, 3‐L stirred‐tank bioreactor at 12°C, 0.46‐vvm aeration rate, 250‐rpm mixing speed, and incident illumination intensity of 77 μE m −2 s −1 . The mean specific growth rate over the exponential phase was 0.185 day −1 and a final cell density of 1083 mg dry cell weight (DCW) L −1 was achieved within 15 days of cultivation from an initial cell density of 200 mg DCW L −1 . The addition of 3500 ppm CO 2 to the aeration gas provided a maximum CO 2 transfer rate of six times the maximum CO 2 consumption rate and stabilized the pH to 8.0 during the light phase of growth, but did not improve biomass productivity. © 1996 John Wiley & Sons, Inc.