Premium
Simultaneous saccharification and extractive fermentation of cellulosic substrates
Author(s) -
Moritz John W.,
Duff Sheldon J. B.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19960305)49:5<504::aid-bit3>3.0.co;2-n
Subject(s) - fermentation , chemistry , hydrolysis , cellulosic ethanol , cellulase , aqueous solution , ethanol , aqueous two phase system , product inhibition , chromatography , enzymatic hydrolysis , reducing sugar , cellulose , pulp and paper industry , sugar , organic chemistry , enzyme , non competitive inhibition , engineering
Alcohol fermentation has traditionally been carried out in aqueous environments because of the ready solubility of reactant (sugar) and product (ethanol). However, extraction of the product ethanol into a nonmiscible phase can result in kinetic benefits due to reduced inhibition of the fermentation reactions. In this study, we report the development of a novel simultaneous saccharification and extractive fermentation (SSEF) process. Ethanol productivity was increased by up to 65% over conventional (nonextractive) fed‐batch simultaneous saccharification systems when calculated on the basis of aqueous phase volume. The amount of water required for SSEF reactions was dramatically reduced from that required for conventional SSF. In batch SSEF reactors with 2.5% aqueous phase, 50% conversion of 25% (aqueous phase concentration) Solka Floc could be achieved in 48 h using 2 FPU/g cellulase. © 1996 John Wiley & Sons, Inc.