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Genetic engineering to contain the Vitreoscilla hemoglobin gene enhances degradation of benzoic acid by Xanthomonas maltophilia
Author(s) -
Liu ShieChau,
Webster Dale A.,
Wei MeiLing,
Stark Benjamin C.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19960105)49:1<101::aid-bit13>3.0.co;2-l
Subject(s) - strain (injury) , xanthomonas , benzoic acid , stenotrophomonas maltophilia , microbiology and biotechnology , biology , bacteria , chemistry , gene , biochemistry , genetics , pseudomonas aeruginosa , anatomy
Xanthomonas maltophilia was transformed with the gene encoding Vitreoscilla (bacterial) hemoglobin, vgb , and the growth of the engineered strain was compared with that of the untransformed strain using benzoic acid as the sole carbon source. In general, growth of the engineered strain was greater than that of the untransformed strain; this was true for experiments using both overnight cultures and log phase cells as inocula, but particularly for the latter. In both cases the engineered strain was also more efficient than the untransformed strain in converting benzoic acid into biomass. © 1996 John Wiley & Sons, Inc.