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The position of the LysN ε H 2 ‐grafted antigens along the sequential oligopeptide carrier, Ac‐(Aib‐Lys‐Aib‐Gly) n (SOC n ‐II), influences the antibody recognition: Application to the Sm main autoimmune epitope
Author(s) -
Alexopoulos Charalampos,
Tsikaris Vassilios,
Rizou Catherina,
SakarellosDaitsiotis Maria,
Sakarellos Constantinos,
Cung Manh Thong,
Marraud Michel,
Vlachoyiannopoulos Panayiotis G.,
Moutsopoulos Haralampos M.
Publication year - 2000
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/(sici)1097-0282(200007)54:1<1::aid-bip10>3.0.co;2-7
Subject(s) - epitope , oligopeptide , chemistry , antigen , moiety , peptide , stereochemistry , autoantibody , antibody , microbiology and biotechnology , biochemistry , immunology , biology
A sequential oligopeptide carrier of antigenic peptides is presented, incorporating two Aib residues in each repetitive moiety: Ac–(Aib–Lys–Aib–Gly) n (SOC n ‐II; n = 2–4). The conformational study, by 1 H‐nmr, CD, and Fourier transform ir spectroscopy, indicated that the SOC n ‐II carrier displays a pronounced 3 10 ‐helix, compared to the Ac–(Lys–Aib–Gly) n (SOC n ‐I) carrier of the same approximately backbone length, previously reported. One of the dominant autoimmune epitopes of the Sm and U1RNP cellular components, the PPGMRPP sequence, was coupled to the Lys‐N ε H 2 groups of the SOC n ‐II carrier and used as antigenic substrate for detecting anti‐Sm/U1RNP autoantibodies in ELISA assays. Anti‐Sm antibodies are highly specific for systemic lupus erythematosus, while anti‐U1RNP are specific for mixed connective tissue disease. The anti‐(PPGMRPP) 5 ‐SOC n ‐II ELISA was compared with the anti‐(PPGMRPP) n –SOC n ‐I ELISA, provided that both antigenic substrates possess the same amount of the epitope replicates. The significance of the lysine positions along the oligopeptide backbone of the carrier for a favorable antibody recognition of the anchored antigens is also examined. © 2000 John Wiley & Sons, Inc. Biopoly 54: 1–10, 2000