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Low frequency vibrations and structural characterization of a murine IgG2a monoclonal antibody studied by Raman and IR spectroscopies
Author(s) -
Picquart M.,
HaroPoniatowski E.,
Morhange J. F.,
Jouanne M.,
Kanehisa M.
Publication year - 2000
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/(sici)1097-0282(20000405)53:4<342::aid-bip6>3.0.co;2-m
Subject(s) - raman spectroscopy , chemistry , monoclonal antibody , characterization (materials science) , molecule , spectral line , vibration , antibody , molecular physics , analytical chemistry (journal) , nuclear magnetic resonance , crystallography , nanotechnology , optics , immunology , materials science , chromatography , physics , biology , organic chemistry , quantum mechanics , astronomy
The Raman and ir spectra of a murine IgG2a monoclonal antibody molecule are reported. In accordance with previous studies on immunoglobulins, the secondary structure is predominantly of the β‐sheet type. The low frequency region of the Raman spectrum was also analyzed in detail. A structured band with two maxima near 43 and 94 cm −1 was observed. This band has been attributed to vibrations of elastic body. The results are interpreted using a simple elastic model. © 2000 John Wiley & Sons, Inc. Biopoly 53: 342–349, 2000