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Physicochemical characterization of generation 5 polyamidoamine dendrimers
Author(s) -
Nourse Amanda,
Millar David B.,
Minton Allen P.
Publication year - 2000
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/(sici)1097-0282(20000405)53:4<316::aid-bip4>3.0.co;2-j
Subject(s) - dendrimer , chemistry , characterization (materials science) , nanotechnology , organic chemistry , materials science
The dispersity, size, and self‐interaction of generation 5 polyamidoamine dendrimeric polymers with different terminal groups (surfaces) were characterized using several physicochemical techniques. Amino‐surface dendrimers form oligomeric aggregates in aqueous solution, even in the presence of high salt concentrations (0.6 M sodium phosphate). In contrast, the hydroxyl‐surface polymer G5‐OH behaves as a single homogeneous (or paucidisperse) species at low concentration. Measurements of density increment and the sedimentation and diffusion coefficients of G5‐OH suggest a more swollen, porous structure than a globular protein of comparable mass. Measurements of the concentration dependence of sedimentation equilibrium of G5‐OH in pH 7.2 phosphate buffer indicate the presence of significant electrostatic repulsion overlaid on weakly attractive interactions, leading to the formation of nonspecific aggregates at sufficiently high dendrimer concentration. © 2000 John Wiley & Sons, Inc. Biopoly 53: 316–328, 2000