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Hydroxyl group interactions in polysaccharides: A deuterium‐induced differential isotope shift 13 C‐NMR investigation
Author(s) -
Bosco M.,
Picotti F.,
Radoicovich A.,
Rizzo R.
Publication year - 2000
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/(sici)1097-0282(200003)53:3<272::aid-bip7>3.0.co;2-s
Subject(s) - chemistry , glycosidic bond , polysaccharide , deuterium , intramolecular force , kinetic isotope effect , solvation , hydrogen bond , proton nmr , computational chemistry , stereochemistry , solvent , organic chemistry , molecule , physics , quantum mechanics , enzyme
The secondary isotope shift in 13 C‐nmr spectra in water was used to obtain information on the interactions of hydroxyl groups with their environment in polysaccharides. Specifically, the possibility of detecting the preference of intramolecular hydrogen bonding with respect to solvation was investigated. Different aliphatic alcohols were studied in both protic and aprotic solvents in order to obtain reference systems. The polysaccharides investigated were selected so as to include both different types of glycosidic linkages and different conformational properties of the polymeric chain. In addition to polysaccharides, β‐cyclodextrin and inulin were also investigated. The experiments demonstrated that isotope shift data can advantageously contribute to the understanding of the conformational properties of polysaccharides and in particular, in setting up of constraints in molecular modeling calculations. © 2000 John Wiley & Sons, Inc. Biopoly 53: 272–280, 2000