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In situ Raman microspectroscopic identification and localization of carotenoids: Approach to monitoring of UV‐B irradiation stress on antarctic fungus
Author(s) -
Arcangeli Caterina,
Cannistraro Salvatore
Publication year - 2000
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/(sici)1097-0282(2000)57:3<179::aid-bip6>3.0.co;2-4
Subject(s) - raman spectroscopy , chemistry , in situ , pigment , carotenoid , irradiation , raman microspectroscopy , spore , fungus , analytical chemistry (journal) , photochemistry , biochemistry , chromatography , optics , botany , organic chemistry , physics , nuclear physics , biology
The in situ Raman microspectroscopic properties of an Antarctic fungus are investigated to assess the nature and the spatial localization of the main chromophores and to study their spectral changes under enhanced UV‐B irradiation. The Raman spectroscopic features of spores in situ are consistent with those of carotenoid‐like pigments. In particular, the Raman shifts seem to be related either to the frequency modes of long conjugated double‐bond carotenoids or to protein bound β‐carotene. The spectroscopic analysis at different spore depths clearly shows the strongest Raman signal arises from cell wall and membrane structures. The intensity of such a signal shows a drastic reduction upon UV‐B irradiation without any significant frequency change. The use of Raman microspectroscopy for nondestructively monitoring the UV‐B effects on Arthrobotrys ferox spores is also discussed. © 2000 John Wiley & Sons, Inc. Biopolymers (Biospectroscopy) 57: 179–186, 2000