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Raman spectroscopy of model membrane monolayers of dipalmitoylphosphatidic acid at the air–water interface using surface enhancement from buoyant thin silver films
Author(s) -
Pemberton Jeanne E.,
Chamberlain James R.
Publication year - 2000
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/(sici)1097-0282(2000)57:2<103::aid-bip7>3.0.co;2-t
Subject(s) - monolayer , chemistry , raman spectroscopy , phosphatidic acid , layer (electronics) , membrane , raman scattering , analytical chemistry (journal) , chemical engineering , thin film , nanotechnology , chromatography , organic chemistry , optics , materials science , phospholipid , biochemistry , physics , engineering
A novel method for the acquisition of surface enhanced Raman (SER) spectra of model membranes of dipalmitoylphosphatidic acid (DPPA) in Langmuir layers at the air–water interface is reported. The approach is based on the electrochemical formation of a buoyant thin layer of coalesced silver colloids in the vicinity of the phosphatidic acid head groups at the interface. This Ag layer is an excellent platform for SER scattering, which shows the spectral features from all parts of the molecule and water between the Ag surface and the DPPA layer. The observation of the spectral response from the phosphatidic acid head groups is of particular significance, allowing insight into their chemical state and orientation at the air–water interface. © 2000 John Wiley & Sons, Inc. Biopolymers (Biospectroscopy) 57: 103–116, 2000