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Free solution mobility of oligomeric DNA
Author(s) -
Mohanty Udayan,
Stellwagen Nancy C.
Publication year - 1999
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/(sici)1097-0282(199903)49:3<209::aid-bip2>3.0.co;2-7
Subject(s) - chemistry , electrophoresis , ionic bonding , oligomer , capillary electrophoresis , electric field , relaxation (psychology) , chemical physics , ionic strength , dna , analytical chemistry (journal) , chromatography , polymer chemistry , aqueous solution , organic chemistry , ion , biochemistry , social psychology , psychology , physics , quantum mechanics
The free solution electrophoretic mobility of a charged oligomer in an ionic solvent that approximately takes into account relaxation field effects, screening of the velocity field, and the hydrodynamic interactions resulting from motions of the charges due to an electric field is described. For double‐stranded DNA, the free solution electrophoretic mobility under ionic strengths determined by the buffer and pH conditions relevant to capillary electrophoresis increases with increasing molecular weight up to few hundred base pairs. © 1999 John Wiley & Sons, Inc. Biopoly 49: 209–214, 1999