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Raman spectroscopic measurements in self‐pressurized aqueous solutions above 100°C: The melting of poly(G) and poly(G) · poly(C)
Author(s) -
Mercier Pascal,
Carrier Virginie,
Roy Simon,
Savoie Rodrigue
Publication year - 1999
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/(sici)1097-0282(199901)49:1<21::aid-bip3>3.0.co;2-y
Subject(s) - chemistry , aqueous solution , raman spectroscopy , tetramethylammonium , polynucleotide , polymer chemistry , perchlorate , bromide , perchloric acid , inorganic chemistry , organic chemistry , ion , physics , optics , biochemistry
We have studied by Raman spectroscopy the thermal behavior of associated polyguanylic acid [poly(G)] and polyguanylic–polycytidylic acid [poly(G) · poly(C)] in self‐pressurized aqueous solutions contained in sealed capillary tubes. The associated polynucleotides were found to be very resistant to heat, but evidence of thermal degradation was observed after melting of the helical structures. The cooperative melting transition of the four‐stranded complex of poly(G) was located at 141°C in 0.5 M KCl, 135°C in 0.5 M NaCl, 129°C in 0.5 M LiCl, 123°C in 0.1 M tetramethylammonium perchlorate, and 105°C in 0.1 M tetraethylammonium bromide solutions. The transition was observed at 130°C in poly(G) · poly(C) (in 0.5 M NaCl). The results in this case show that a four‐stranded poly(G) complex is formed following the melting of the double helix. © 1999 John Wiley & Sons, Inc. Biopoly 49: 21–28, 1999