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UV resonance Raman spectroscopy of DNA and protein constituents of viruses: Assignments and cross sections for excitations at 257, 244, 238, and 229 nm
Author(s) -
Wen Zai Qing,
Thomas George J.
Publication year - 1998
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/(sici)1097-0282(199803)45:3<247::aid-bip7>3.0.co;2-r
Subject(s) - chemistry , resonance (particle physics) , excitation , raman spectroscopy , excitation wavelength , dna , ultraviolet , raman scattering , aromatic amino acids , resonance raman spectroscopy , nucleoprotein , wavelength , amino acid , spectroscopy , analytical chemistry (journal) , crystallography , atomic physics , biochemistry , optics , chromatography , optoelectronics , materials science , engineering , physics , quantum mechanics , electrical engineering
Ultraviolet resonance Raman (UVRR) spectra of H 2 O and D 2 O solutions of the nucleoside (dA, dG, dC, dT) and aromatic amino acid (Phe, Trp, Tyr) constituents of DNA viruses have been obtained with laser excitation wavelengths of 257, 244, 238, and 229 nm. Using the 981 cm −1 marker of Na 2 SO 4 as an internal standard, Raman frequencies and scattering cross sections were evaluated for all prominent UVRR bands at each excitation wavelength. The results show that UVRR cross sections of both the nucleosides and amino acids are strongly dependent on excitation wavelength and constitute sensitive and selective probes of the residues. The results provide a library of UVRR marker bands for structural analysis of DNA viruses and other nucleoprotein assemblies. © 1998 John Wiley & Sons, Inc. Biopoly 45: 247–256, 1998