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X‐ray crystallographic analysis of the hydration of A‐ and B‐form DNA at atomic resolution
Author(s) -
Egli Martin,
Tereshko Valentina,
Teplova Marianna,
Minasov George,
Joachimiak Andrzei,
Sanishvili Ruslan,
Weeks Charles M.,
Miller Russ,
Maier Martin A.,
An Haoyun,
Dan Cook P.,
Manoharan Muthiah
Publication year - 1998
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/(sici)1097-0282(1998)48:4<234::aid-bip4>3.0.co;2-h
Subject(s) - dodecameric protein , chemistry , crystallography , dna , nucleic acid , resolution (logic) , low resolution , crystal structure , diffraction , x ray , high resolution , biochemistry , remote sensing , artificial intelligence , computer science , geology , physics , quantum mechanics , optics
We have determined single crystal structures of an A‐DNA decamer and a B‐DNA dodecamer at 0.83 and 0.95 Å, respectively. The resolution of the former is the highest reported thus far for any right‐handed nucleic acid duplex and the quality of the diffraction data allowed determination of the structure with direct methods. The structures reveal unprecedented details of DNA fine structure and hydration; in particular, we have reexamined the overall hydration of A‐ and B‐form DNA, the distribution of water around phosphate groups, and features of the water structure that may underlie the B to A transition. © 2000 John Wiley & Sons, Inc. Biopoly 48: 234–252, 1998