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Water and monovalent ions in the minor groove of B‐DNA oligonucleotides as seen by NMR
Author(s) -
Halle Bertil,
Denisov Vladimir P.
Publication year - 1998
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/(sici)1097-0282(1998)48:4<210::aid-bip3>3.0.co;2-y
Subject(s) - chemistry , oligonucleotide , ion , minor groove , dna , nuclear magnetic resonance spectroscopy , relaxation (psychology) , nuclear overhauser effect , crystallography , nuclear magnetic resonance , stereochemistry , organic chemistry , biochemistry , social psychology , psychology , physics
During the past 8 years, two complementary nmr techniques—magnetic relaxation dispersion and nuclear Overhauser effect spectroscopy—have been applied extensively to the study of water and monovalent ions in the minor groove of B‐DNA oligonucleotides in solution. In this review, the possibilities and limitations of the two methods are outlined, with emphasis on the interpretational steps whereby molecular‐level information is extracted from the primary data. The results on sequence‐dependent hydration and ion–DNA interactions obtained so far by these methods is summarized and critically assessed. The nmr results are also compared with structural data from x‐ray crystallography. © 2000 John Wiley & Sons, Inc. Biopoly 48: 210–233, 1998