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Use of 13 C tags with specifically 15 N‐labeled DNA and RNA
Author(s) -
Abad JoséLuis,
Shallop Anthony J.,
Gaffney Barbara L.,
Jones Roger A.
Publication year - 1998
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/(sici)1097-0282(1998)48:1<57::aid-bip6>3.0.co;2-b
Subject(s) - chemistry , rna , dna , monomer , nucleic acid , adenosine , base (topology) , stereochemistry , biochemistry , organic chemistry , polymer , gene , mathematical analysis , mathematics
We describe methods to introduce 13 C specifically to the C2 or C8 positions of 15 N‐multilabeled purines, thereby permitting “ 13 C tagging” of all base nitrogens except the amino group of adenosine. These procedures permit the incorporation of two or more 15 N‐multilabeled monomers into a given DNA or RNA fragment, with and without appropriate 13 C tags. This approach increases the number of 15 N‐nmr resonances that can be unambiguously distinguished and thus the amount of information from a single synthesis and a single nmr experiment. © 1998 John Wiley & Sons, Inc. Biopoly 48: 57–63, 1998