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Modified oligoribonucleotides as site‐specific probes of RNA structure and function
Author(s) -
Earnshaw David J.,
Gait Michael J.
Publication year - 1998
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/(sici)1097-0282(1998)48:1<39::aid-bip5>3.0.co;2-a
Subject(s) - ribozyme , chemistry , rna , ligase ribozyme , nucleotide , nucleic acid structure , function (biology) , base pair , computational biology , biochemistry , combinatorial chemistry , dna , gene , biology , genetics
Modified nucleotides can be incorporated site specifically into RNA by the use of total chemical synthesis as well as by use of a variety of recombinant RNA techniques. The range of nucleotide analogues includes modifications to base, sugar, and phosphate for structure–function analysis and for cross‐linking studies as well as to answer specific mechanistic questions in RNA catalysis. We describe how RNA containing site‐specific modifications are prepared, concentrating in particular on routes involving chemically synthesized oligoribonucleotides, and give examples of their application in studies of the hammerhead and hairpin ribozymes. © 1998 John Wiley & Sons, Inc. Biopoly 48: 39–55, 1998