Premium
Measuring the thermodynamics of RNA secondary structure formation
Author(s) -
SantaLucia John,
Turner Douglas H.
Publication year - 1997
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/(sici)1097-0282(1997)44:3<309::aid-bip8>3.0.co;2-z
Subject(s) - chemistry , sequence (biology) , rna , thermodynamics , simple (philosophy) , nucleic acid secondary structure , statistical physics , protein secondary structure , physics , biochemistry , philosophy , epistemology , gene
The thermodynamics of RNA secondary structure formation in small model systems provides a database for predicting RNA structure from sequence. Methods for making these measurements are reviewed with emphasis on optical methods and treatment of experimental errors. Analysis of experimental results in terms of simple nearest‐neighbor models is presented. Some measured sequence dependences of non‐Watson‐Crick motifs are discussed. © 1998 John Wiley & Sons, Inc. Biopoly 44: 309–319, 1997