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Physicochemical studies on xylinan (acetan). I. Characterization by G permeation chromatography on sepharose Cl‐2B coupled with static light scattering and viscometry
Author(s) -
Berth Gisela,
Dautzenberg Herbert,
Christensen Bjørn E.,
Rother Gudrun,
Smidsrød Olav
Publication year - 1996
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/(sici)1097-0282(199611)39:5<709::aid-bip9>3.0.co;2-#
Subject(s) - chemistry , multiangle light scattering , dispersity , persistence length , molar mass , viscometer , fractionation , chromatography , gel permeation chromatography , sepharose , light scattering , analytical chemistry (journal) , static light scattering , scattering , polymer chemistry , thermodynamics , polymer , optics , viscosity , organic chemistry , physics , enzyme
Laboratory‐made samples of the polysaccharide xylinan (acetan) were fractionated on Sepharose Cl‐2B using 0.1 M NaCl as eluant. The weight average molar masses and intrinsic viscosities were estimated in the fractions by multiangle laser light scattering (off‐line) and capillary viscometry, respectively. The Mark‐Houwink‐Sakurada plot was found to be indicative of semiflexible coils ( a = 0.90). The angular dependence of scattered light was interpreted by fitting with theoretically calculated “Master Curves” in terms of a wormlike chain model. The ambiguity of the interpretation of scattering curves owing to the overlapping effects of chain stiffness and polydispersity is discussed in detail. The experimental data is found to be consistent with a persistence length of L p = 100 nm. The main proportion consists of double‐stranded chains (consistent with a robust double‐helix), but single‐ and multistranded chains also are present. Our results suggest a fractionation according to the contour length rather than the molar mass. © 1996 John Wiley & Sons, Inc.