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An NMR self‐diffusion study of the interactions between spermidine and oligonucleotides
Author(s) -
Andreasson Bo,
Nordenskiöld Lars,
Braunlin William H.
Publication year - 1996
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/(sici)1097-0282(199604)38:4<505::aid-bip6>3.0.co;2-x
Subject(s) - spermidine , chemistry , diffusion , oligonucleotide , self diffusion , pulsed field gradient , analytical chemistry (journal) , chromatography , organic chemistry , molecule , biochemistry , thermodynamics , dna , enzyme , physics , self service , marketing , business
Self‐diffusion coefficients have been determined by pulsed field gradient nmr methods for spermidine in solutions of the oligonucleotides d(GC) 4 and d(GGAATTCC). The self‐diffusion behavior of spermidine in solution of d(GC) 4 is very similar to that observed previously for methylspermidine (completely N‐methylated spermidine). Moreover, the self‐diffusion behaviors of spermidine in solutions of d(GC) 4 and d(GGAATTCC) are also quite similar, indicating that there is no significant influenceon on self‐diffusion of oligonucleotide base composition. Furthermore, self‐diffusion coefficients of the oligonucleotide d(GC) 8 show only a small dependence on oligonucleotide concentration, and no measurable dependence on sodium ion or magnesium ion concentration. © 1996 John Wiley & Sons, Inc.