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Rapid and sensitive determination of paclitaxel (Taxol®) in environmental samples by high‐performance liquid chromatography tandem mass spectrometry
Author(s) -
Sottani C.,
Turci R.,
Micoli G.,
Fiorentino M. L.,
Minoia C.
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(20000530)14:10<930::aid-rcm968>3.0.co;2-4
Subject(s) - chemistry , chromatography , extraction (chemistry) , detection limit , mass spectrometry , paclitaxel , liquid chromatography–mass spectrometry , tandem mass spectrometry , selected reaction monitoring , ethyl acetate , gas chromatography/tandem mass spectrometry , medicine , surgery , chemotherapy
A new, rapid and sensitive method for the quantitative determination of paclitaxel (Taxol®) in environmental samples is developed and validated. This highly sensitive and specific method was successfully applied to an environmental monitoring study for identifying occupational exposure situations to anticancer drugs. After addition of an internal standard, 2′‐methylpaclitaxel, aqueous extraction of wipe samples or air filters followed by a single‐step liquid‐liquid extraction with ethyl acetate was performed. Extraction efficiency averaged 90%. The method is based on analysis by liquid chromatography with tandem mass spectrometry in selected reaction monitoring mode of environmental samples from the preparation and administration of cytostatic agents. The assay is linear (r = 0.998) in the range 2–64 ng on filters (air samples), and 20–320 ng on wipe samples. The accuracy of the assay is always ≤20% for both matrices, and the intra‐ and interday assay variability of paclitaxel ranged from 7 to 19% and 10 to 14%, respectively. The lower limit of quantification is assessed at 2 ng on filter and 10 ng on wipe samples. Copyright © 2000 John Wiley & Sons, Ltd.