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Investigation of unknown related substances in commercial erythromycin samples with liquid chromatography/mass spectrometry
Author(s) -
Govaerts Cindy,
Chepkwony Hezekiah Kibet,
Schepdael Ann Van,
Roets Eugène,
Hoogmartens Jos
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(20000530)14:10<878::aid-rcm959>3.0.co;2-e
Subject(s) - chemistry , chromatography , mass spectrometry , electrospray , liquid chromatography–mass spectrometry , impurity , ion trap , analytical chemistry (journal) , organic chemistry
Abstract A selective reversed phase liquid chromatography/mass spectrometry (LC/MS n ) method is described for the identification of erythromycin impurities and related substances in commercial erythromycin samples. Mass spectral data are acquired on a LCQ ion trap mass spectrometer equipped with an electrospray interface operated in positive ion mode. The LCQ is ideally suited for identification of impurities and related substances because it provides on‐line LC/MS n capability. Compared with UV detection, this hyphenated LC/MS n technique provides as a main advantage efficient identification of novel substances without time‐consuming isolation and purification procedures. Using this method four novel related substances were identified in commercial samples. Copyright © 2000 John Wiley & Sons, Ltd.