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Detection of intact hemoglobin from aqueous solution with laser desorption mass spectrometry
Author(s) -
Wattenberg Andreas,
Sobott Frank,
Brutschy Bernhard
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(20000530)14:10<859::aid-rcm956>3.0.co;2-m
Subject(s) - chemistry , mass spectrometry , desorption , surface enhanced laser desorption/ionization , chromatography , analytical chemistry (journal) , sample preparation in mass spectrometry , soft laser desorption , aqueous solution , matrix assisted laser desorption electrospray ionization , laser , ionization , protein mass spectrometry , ion , matrix assisted laser desorption/ionization , tandem mass spectrometry , adsorption , electrospray ionization , organic chemistry , optics , physics
Laser induced liquid beam ionization/desorption mass spectrometry (LILBID‐MS) is a new desorption method recently developed in our laboratory. This method allows ions to be desorbed directly from the liquid phase into the high‐vacuum region of a mass spectrometer. This method has now been applied to the detection of noncovalent protein‐protein complexes. The example given in this paper is the quartenary complex of human hemoglobin. For the first time, the intact hemoglobin could be detected by laser desorption mass spectrometry. Furthermore, evidence for the specificity of the complex is given. Copyright © 2000 John Wiley & Sons, Ltd.