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Differentiation of enantiomers using matrix‐assisted laser desorption/ionization mass spectrometry
Author(s) -
So M. P.,
Wan T. S. M.,
Dominic Chan T.W.
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(20000430)14:8<692::aid-rcm934>3.0.co;2-j
Subject(s) - chemistry , enantiomer , mass spectrometry , chromatography , ionization , deuterium , matrix assisted laser desorption/ionization , enantiomeric excess , desorption , matrix (chemical analysis) , matrix assisted laser desorption electrospray ionization , cyclodextrin , organic chemistry , enantioselective synthesis , ion , physics , quantum mechanics , adsorption , catalysis
Matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) has successfully been used to differentiate pseudo‐enantiomeric (isotopically labelled) amino acids by using cyclodextrin as complexing host. By using different pseudo‐enantiomeric mixtures (i.e. R Dn + S; and R + S Dn ), it has been demonstrated that the preference of cyclodextrin for S ‐enantiomers is not due to the size differences caused by the hydrogen/deuterium substitution. It is postulated that this method can be extended to differentiate enantiomers (and determine enantiomeric excess) by using a pair of enantiomeric hosts, as demonstrated previously using other ionization techniques, but with much higher sensitivity. Copyright © 2000 John Wiley & Sons, Ltd.