Premium
Effects of ion mode and matrix additives in the identification of bacteria by intact cell mass spectrometry
Author(s) -
Evason David J.,
Claydon Martin A.,
Gordon Derek B.
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(20000430)14:8<669::aid-rcm932>3.0.co;2-7
Subject(s) - chemistry , formic acid , mass spectrometry , chromatography , crown ether , matrix (chemical analysis) , adduct , analytical chemistry (journal) , sample preparation in mass spectrometry , matrix assisted laser desorption/ionization , mass spectrum , ion , desorption , electrospray ionization , organic chemistry , adsorption
Protocols for the identification of bacterial cells by intact cell matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry (ICM‐TOFMS) are presented. A mass range of 500 to 10 000 m/z is used. The use of formic acid and the crown ether 1, 4, 7, 10, 13, 16‐hexaoxacyclooctadecane (18‐crown‐6) is described. Crown ether is useful for removing metal ion adducts, which degrade spectral purity, and formic acid promotes positive ions, improves spectral signal, and, hence, increases identification certainty. Copyright © 2000 John Wiley & Sons, Ltd.