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Chromatographic aspects in high throughput liquid chromatography/mass spectrometry
Author(s) -
Pereira Luisa,
Ross Paul,
Woodruff Mark
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(20000315)14:5<357::aid-rcm889>3.0.co;2-b
Subject(s) - chemistry , chromatography , mass spectrometry , liquid chromatography–mass spectrometry , column (typography) , chromatography column , column chromatography , analytical chemistry (journal) , structural engineering , connection (principal bundle) , engineering
Important parameters to consider when developing a liquid chromatography/mass spectrometry (LC/MS) method are buffer type and concentration, and column geometry. In the work presented here the choice of buffer for the analysis of basic compounds using a polar embedded phase (HyPURITY™ ADVANCE) is illustrated for the analysis of tricyclic antidepressants. Method transfer from a 4.6 mm i.d. column to a 2.1 mm i.d. column is demonstrated for the analysis of triazines and anabolic steroids and their metabolites, with no change in selectivity and with added speed of analysis. Analysis of eight β‐blockers is achieved in 65 seconds by using a short 30 × 4.6 mm C18 column. Copyright © 2000 John Wiley & Sons, Ltd.