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High throughput on‐line solid phase extraction/tandem mass spectrometric determination of paclitaxel in human serum
Author(s) -
Schellen Anniek,
Ooms Bert,
Gils Mark van,
Halmingh Otto,
Vlis Erik van der,
Lagemaat Dick van de,
Verheij Elwin
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(20000229)14:4<230::aid-rcm872>3.0.co;2-j
Subject(s) - chemistry , solid phase extraction , chromatography , tandem , paclitaxel , tandem mass spectrometry , extraction (chemistry) , throughput , mass spectrometry , medicine , cancer , materials science , composite material , telecommunications , computer science , wireless
The feasibility of high throughput on‐line solid phase extraction/tandem mass spectrometry (SPE/MS/MS) is tested for target analysis of paclitaxel in human serum. The use of a dual Prospekt system, with parallel SPE and elution directly to the mass spectrometer, resulted in a cycle time of 80 seconds for the entire, fully automated assay. The assay proved to be linear from 1 to 1000 ng/mL. Cartridges packed with small sorbent particles functioned both as SPE cartridges and as short analytical columns. Copyright © 2000 John Wiley & Sons, Ltd.