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Determination of disulfide bonds in highly bridged α‐dendrotoxin by matrix‐assisted laser desorption/ionization mass spectrometry
Author(s) -
Belva Hélène,
Valois Chrystel,
Lange Catherine
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(20000229)14:4<224::aid-rcm871>3.0.co;2-b
Subject(s) - chemistry , mass spectrometry , matrix assisted laser desorption/ionization , desorption , ionization , sample preparation in mass spectrometry , matrix (chemical analysis) , chromatography , matrix assisted laser desorption electrospray ionization , surface enhanced laser desorption/ionization , analytical chemistry (journal) , electrospray ionization , organic chemistry , ion , adsorption
A methodology which combines tryptic digestions, 1,4‐dithiothreitol reduction, and matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐TOF‐MS) allows verification of the location of the disulfide bridges in α‐dendrotoxin (C7‐C57, C16‐C40 and C32‐C53). Copyright © 2000 John Wiley & Sons, Ltd.