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Mass spectrometric study of randomly methylated β‐cyclodextrins using ionspray, atmospheric pressure chemical ionization and matrix‐assisted laser desorption/ionization
Author(s) -
Mercier J.Ph.,
Debrun J. L.,
Dreux M.,
Elfakir C.,
Hakim B.
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(20000130)14:2<68::aid-rcm835>3.0.co;2-w
Subject(s) - chemistry , atmospheric pressure chemical ionization , mass spectrometry , ionization , desorption , chemical ionization , atmospheric pressure laser ionization , analytical chemistry (journal) , atmospheric pressure , matrix assisted laser desorption electrospray ionization , chromatography , matrix assisted laser desorption/ionization , matrix (chemical analysis) , ambient ionization , photoionization , organic chemistry , adsorption , ion , oceanography , geology
Mixtures of methylated β‐cyclodextrins were characterized using three different methods of mass spectrometry: ionspray, atmospheric pressure chemical ionization (APCI) and matrix‐assisted laser desorption/ionization (MALDI). Each of these methods allows a fast and simple determination of the degree of substitution, and can provide evidence for differences in the methylation of batches which have very similar global degrees of substitution. The three methods are in good qualitative agreement, but there are systematic differences in the quantitative results for the percentages of the various methylated molecules present in a batch. This is attributed to ionization yields which increase with the number of methyl groups, with different slopes for the different methods. Copyright © 2000 John Wiley & Sons, Ltd.