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Electrospray ionisation Fourier‐transform ion cyclotron resonance mass spectrometry of dynamic combinatorial libraries
Author(s) -
Poulsen SallyAnn,
Gates Paul J.,
Cousins Graham R. L.,
Sanders Jeremy K. M.
Publication year - 2000
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(20000115)14:1<44::aid-rcm832>3.0.co;2-6
Subject(s) - fourier transform ion cyclotron resonance , chemistry , mass spectrometry , electrospray ionization , tandem mass spectrometry , top down proteomics , protein mass spectrometry , electrospray , ion cyclotron resonance , analytical chemistry (journal) , ion , combinatorial chemistry , chromatography , cyclotron , organic chemistry
The use of electrospray ionisation Fourier‐transform ion cyclotron resonance tandem mass spectrometry (ESI‐FTICR‐MS/MS) for the analysis of dynamic combinatorial libraries (DCLs) of pseudo‐peptide macrocyclic hydrazone oligomers is presented. The design of library building blocks results in mixtures of compounds with greater diversity than libraries generated by conventional combinatorial chemistry and so presents increased demands for analysis. The extended capabilities of the FTICR technique, specifically selective ion trapping, sensitivity, high resolution and mass accuracy over a broad mass range, are compatible with these increased demands and, most importantly, without the need for chromatography. Preliminary studies on the sequencing of cyclic oligomers and confirmation of the presence of sequence isomers are presented. These studies highlight the potential of FTICR‐MS as a superior technique for the analysis of combinatorially generated compounds. Copyright © 2000 John Wiley & Sons, Ltd.