Premium
Use of meso ‐ tetrakis(pentafluorophenyl)porphyrin as a matrix for low molecular weight alkylphenol ethoxylates in laser desorption/ ionization time‐of‐flight mass spectrometry
Author(s) -
Ayorinde F. O.,
Hambright P.,
Porter T. N.,
Keith Q. L.
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19991230)13:24<2474::aid-rcm814>3.0.co;2-0
Subject(s) - chemistry , mass spectrometry , analytical chemistry (journal) , mass spectrum , porphyrin , desorption , nonylphenol , matrix assisted laser desorption/ionization , matrix (chemical analysis) , ion , molecule , ionization , time of flight mass spectrometry , chromatography , organic chemistry , adsorption , environmental chemistry
The use of UV‐absorbing molecules as matrices in matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) is well documented. The matrices that are currently used have low molecular weights (<300 Da) and thus, for a typical MALDI‐TOF spectrum, the low‐mass range ( m/z 100–500) is dominated by matrix ions. Consequently, the applications of MALDI‐TOFMS have been restricted mostly to the analysis of high molecular weight analytes. This report demonstrates the use of meso ‐tetrakis(pentafluorophenyl)porphyrin (F20TPP, MW 974.57) as a matrix in the MALDI‐TOF mass spectrometric analysis of some commercial nonylphenol ethoxylates (4‐(C 9 H 19 )‐C 6 H 4 ‐(OCH 2 CH 2 ) n ‐OH), in which the ethoxymer ion distribution ranges from 331–771 Da. When F20TPP was used without a sodium ion dopant, there were no MALDI signals for the ethoxylates. However, addition of sodium acetate to the sample produced MALDI spectra in which the ethoxymer molecules were sodiated to form [M + Na] + ions. A comparison of the mass spectrometric data with those obtained when α‐cyano‐4‐hydroxycinnamic acid (CHCA) was used as the matrix indicated that the F20TPP‐induced spectra provided comparable data, with the advantage of having less matrix interference in the low‐mass range ( m/z 100–500). Thus, the use of F20TPP and similar porphyrins may provide the means to apply MALDI‐TOF to the analysis of low molecular weight molecules with minimum interference from matrix signals. © 1999 John Wiley & Sons, Ltd.