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Application of liquid chromatography atmospheric pressure chemical ionization tandem mass spectrometry in the quantitative analysis of glyburide (glibenclamide) in human plasma
Author(s) -
Ramos L.,
Bakhtiar R.,
Tse F.
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19991230)13:24<2439::aid-rcm809>3.0.co;2-c
Subject(s) - chemistry , glibenclamide , atmospheric pressure chemical ionization , chromatography , protein precipitation , liquid chromatography–mass spectrometry , mass spectrometry , tandem mass spectrometry , acetonitrile , chemical ionization , analytical chemistry (journal) , ionization , diabetes mellitus , ion , organic chemistry , medicine , endocrinology
Glyburide (glibenclamide) is widely prescribed in the treatment of Type II diabetes. A validated liquid chromatography atmospheric pressure chemical ionization tandem mass spectrometry (LC/APCI‐MS/MS) method for the determination of glyburide is reported. The method uses a stable isotope labeled glyburide as the internal standard. Subsequent to acetonitrile protein precipitation, the supernatant was directly (unfiltered) injected onto the LC column (retention time ∼3 min) for analysis. A lower limit of quantification (LLOQ) of 1.01 ng/mL was attained for the human plasma assay. The method was fast, specific, and exhibited excellent ruggedness. It was successfully applied to the analysis of clinical samples from patients dosed with glyburide. © 1999 John Wiley & Sons, Ltd.