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Laser desorption of DNA oligomers larger than one kilobase from cooled 4‐nitrophenol
Author(s) -
Lin Hua,
Hunter Joanna M.,
Becker Christopher H.
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19991215)13:23<2335::aid-rcm794>3.0.co;2-1
Subject(s) - chemistry , oligomer , desorption , sublimation (psychology) , dna , mass spectrometry , matrix (chemical analysis) , nitrophenol , laser , analytical chemistry (journal) , chromatography , matrix assisted laser desorption/ionization , polymer chemistry , organic chemistry , biochemistry , optics , psychology , physics , adsorption , psychotherapist , catalysis
A unique matrix system consisting mostly of 4‐nitrophenol has shown to be very effective for matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometric analysis of large DNA oligomers when a cooled sample stage was used to prevent the sublimation of this matrix under vacuum. Using this 4‐nitrophenol matrix with UV laser desorption, detection of picomole quantities of DNA oligomers containing up to approximately 800 nucleotides was routinely achieved. The effectiveness of this matrix was further demonstrated by the observation of a double‐stranded DNA oligomer larger than 1000 base pairs, seen as a denatured single‐stranded species, with a molecular ion mass exceeding 300 000 Da. The potential applications of 4‐nitrophenol as a matrix for DNA sizing are discussed. Copyright © 1999 John Wiley & Sons, Ltd.