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Analysis of exogenous dehydroepiandrosterone excretion in urine by gas chromatography/combustion/isotope ratio mass spectrometry
Author(s) -
Ueki Makoto,
Okano Masato
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19991130)13:22<2237::aid-rcm780>3.0.co;2-c
Subject(s) - chemistry , dehydroepiandrosterone , urine , isotope ratio mass spectrometry , excretion , chromatography , gas chromatography , endocrinology , gas chromatography–mass spectrometry , mass spectrometry , medicine , hormone , androgen , biochemistry
A detailed procedure for the analysis of exogenous dehydroepiandrosterone (DHEA) in urine by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) has been established for detecting doping with DHEA. The average δ‐value (parts per thousand difference of 13 C/ 12 C ratio from the isotope ratio standard) of 26 synthetic steroids commercially available was −30.1 ± 2.6, and was significantly lower than that of human endogenous DHEA in urine of the world class athletes who had participated in the XVIIth Olympic Winter Games (−20.3 ± 2.1, n = 446). Although large inter‐individual variations of urinary DHEA excretion were observed following a single oral administration of 50 mg of DHEA, no significant inter‐individual difference was found when the excretion of exogenous DHEA was monitored in terms of δ‐values using GC/C/IRMS; the minimum δ‐values were observed around 6–8 h after the administration, and the values returned to the base level at over 72 h after the dosing. Thus, the deviations in δ‐values of DHEA and its diol metabolites are considered to be conclusive evidence for detecting doping with DHEA. Some successful cases of detection of doping with DHEA from athletes are also reported. Copyright © 1999 John Wiley & Sons, Ltd.