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Direct determination of bisphenol A and nonylphenol in river water by column‐switching semi‐microcolumn liquid chromatography/electrospray mass spectrometry
Author(s) -
Motoyama Akira,
Suzuki Ayako,
Shirota Osamu,
Namba Ryujiro
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19991115)13:21<2204::aid-rcm776>3.0.co;2-9
Subject(s) - chemistry , nonylphenol , chromatography , electrospray , bisphenol a , bisphenol , detection limit , mass spectrometry , high performance liquid chromatography , analytical chemistry (journal) , environmental chemistry , organic chemistry , epoxy
A rapid and sensitive analytical method based on column‐switching semi‐microcolumn high‐performance liquid chromatography (HPLC) with electrospray mass spectrometry was developed for determining trace levels of bisphenol A (2,2‐bis(4‐hydroxyphenyl)propane) and nonylphenol (4‐nonylphenol) in river water. An aliquot of sample solution was directly injected into the precolumn packed with Capcellpak MF‐Ph for sample cleanup and enrichment. The compounds of interest were then transferred to a C‐18 analytical column for main separation through a change in flow path by a programmed switching valve. Bisphenol A, nonylphenol, and interfering substances were satisfactorily separated with a simple gradient elution complete within 35 min. Detection of their deprotonated molecules was conducted in negative ion mode. A reduced flow rate (100 µL/min) optimized for the narrow bore column was found advantageous in obtaining a high degree of sensitivity during electrospray detection. The influence of carrier additives on sensitivity was also examined. This method produced detection limits of 0.5 ng/mL for bisphenol A and 10 ng/mL for nonylphenol (signal‐to‐noise ratio 3). Calibration curves were observed in the range of 2.5–50 ng/mL (r 2 = 0.999) for bisphenol A and 50–500 ng/mL (r 2 = 0.998) for nonylphenol. Recoveries of the compounds from spiked distilled water and river water were 99.7–138.5% with a relative standard deviation (RSD) ranging from 2.2–9.7%. Copyright © 1999 John Wiley & Sons, Ltd.