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Influence of acceleration voltages on relative ion intensities in the post‐source decay fragmentation of isomeric cyclic oligosaccharides by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Yamagaki Tohru,
Nakanishi Hiroshi
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19991115)13:21<2199::aid-rcm775>3.0.co;2-s
Subject(s) - chemistry , fragmentation (computing) , mass spectrometry , ion , ionization , analytical chemistry (journal) , ion source , desorption , acceleration , collision induced dissociation , time of flight mass spectrometry , soft laser desorption , matrix assisted laser desorption/ionization , chromatography , tandem mass spectrometry , adsorption , organic chemistry , physics , computer science , operating system , classical mechanics
The isomeric sugar‐branched cyclodextrin (CD) derivatives of 6‐ O ‐glucosyl‐βCD (G1‐βCD) and 6,6‐di‐ O ‐glucosyl‐αCD (G1,G1‐αCD) were analyzed by matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) mass spectrometry at the different acceleration voltages of 20 and 8 kV. In the post‐source decay (PSD) fragment spectra of G1‐βCD and G1, G1‐αCD under the reduced acceleration voltage 8 kV, the relative intensity ratios of one‐site cleavage ions [M − G1] + to two‐site cleavage ions [M − G2 ∼ 5] + were much larger than at 20 kV. This change in the intensity ratio in the spectra of G1, G1‐αCD was much larger than in the spectra of G1‐βCD. The measurements under the reduced acceleration voltage were useful to distinguish easily between the otherwise very similar PSD fragment spectra of G1‐βCD and G1, G1‐αCD. It was concluded that the acceleration energy is one of the most effective parameters controlling relative ion intensities in PSD, and that distinction between these oligosaccharide isomers is facilitated by manipulation of this parameter. Copyright © 1999 John Wiley & Sons, Ltd.