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Rapid approach to the quantitative determination of Topiramate (2,3:4,5‐bis‐ O ‐(1‐methylethylidene)‐β‐ D ‐fructopyranose sulfamate) in human plasma by liquid–liquid extraction and flow‐injection negative‐ion electrospray mass spectrometry
Author(s) -
Chen Su,
Carvey Paul M.
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19991030)13:20<1980::aid-rcm741>3.0.co;2-c
Subject(s) - chemistry , topiramate , chromatography , electrospray ionization , electrospray , extraction (chemistry) , mass spectrometry , quantitative analysis (chemistry) , analytical chemistry (journal) , neuroscience , epilepsy , biology
Topiramate, a sulfamate‐substituted monosaccharide (2,3:4,5‐bis‐ O ‐(1‐methylethylidene)‐β‐ D ‐fructopyranose sulfamate), is a new antiepileptic drug, which has been approved for adjunctive therapy in adult patients with partial‐onset seizures. Liquid‐liquid extraction followed by flow‐injection negative‐ion electrospray mass spectrometry was evaluated as a means for the quantitative analysis of Topiramate in human plasma. Prednisone (1,4‐pregnadiene‐17‐α,21‐diol‐3,11,20‐trione [15 µg/mL]) was used as the internal standard because its solubility and molecular weight are similar to those of Topiramate. Calibration curves for Topiramate were linear over a range of 1 to 30 µg/mL plasma (signal‐to‐noise ratio >4) and were highly reliable ( r 2 = 0.994). This approach offers several advantages: (i) the extraction of Topiramate from human plasma using chloroform is simple and reproducible; (ii) the quantitative determination of Topiramate, in the presence of an internal standard, by flow‐injection negative‐ion electrospray mass spectrometry with selected‐ion recording, is rapid and accurate and does not require chromatographic separation; (iii) the assay possesses adequate sensitivity (2–25 µg/mL) for the quantitative analysis of Topiramate in plasma from patients. Copyright © 1999 John Wiley & Sons, Ltd.