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Analysis of 9‐fluorenylmethyloxycarbonyl derivatives of catecholamines by high performance liquid chromatography, liquid chromatography/mass spectrometry and tandem mass spectrometry
Author(s) -
Chen Su,
Li Qing,
Carvey Paul M.,
Li Kawan
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990930)13:18<1869::aid-rcm731>3.0.co;2-h
Subject(s) - chemistry , mass spectrometry , electrospray ionization , chromatography , tandem mass spectrometry , atmospheric pressure chemical ionization , mass spectrum , electrospray , liquid chromatography–mass spectrometry , protein mass spectrometry , protonation , chemical ionization , sample preparation in mass spectrometry , collision induced dissociation , high performance liquid chromatography , analytical chemistry (journal) , ionization , ion , organic chemistry
9‐Fluorenylmethyloxycarbonyl (FMOC) derivatives of catecholamines, including dopamine (DA; 3,4‐dihydroxyphenethylamine), norepinephrine (NE; 2‐amino‐1‐(3,4‐dihydroxyphenyl)ethanol) and epinephrine (EPI; 1‐phenyl‐1‐hydroxy‐2‐methylaminopropane) as well as 3,4‐dihydroxybenzylamine (DHBA) have been analyzed using high performance liquid chromatography coupled to fluorometric detection and atmospheric pressure chemical ionization mass spectrometry at low femtomole levels. Structures of the derivatives have also been characterized by liquid chromatography/atmospheric pressure chemical ionization mass spectrometry and electrospray/tandem mass spectrometry. Protonated molecules of FMOC‐DA and FMOC‐DHBA as well as [M + H − H 2 O] + ions of FMOC‐NE and FMOC‐EPI appear in their conventional mass spectra, and abundant fragments characteristic of catecholamines dominated the spectra. Structurally diagnostic ions using several hundred femtomoles of the FMOC derivatives of catecholamine were observed. Collision‐induced dissociation (CID) of electrospray‐generated protonated molecules of FMOC‐DA and FMOC‐DHBA as well as [M + H − H 2 O] + ions of FMOC‐NE and FMOC‐EPI produced diagnostic product ion spectra. Liquid chromatography/atmospheric pressure chemical ionization mass spectrometric analysis of the FMOC derivatives of catecholamines and DHBA should prove useful in the separation and characterization of these compounds from biological materials. Copyright © 1999 John Wiley & Sons, Ltd.