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Chemical reactivity in matrix‐assisted laser desorption/ionization mass spectrometry
Author(s) -
Enjalbal C.,
Sauvagnat B.,
Lamaty F.,
Lazaro R.,
Martinez J.,
Mouchet P.,
Roux F.,
Aubagnac J.L.
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990930)13:18<1775::aid-rcm712>3.0.co;2-u
Subject(s) - chemistry , mass spectrometry , sample preparation in mass spectrometry , reactivity (psychology) , matrix assisted laser desorption/ionization , fast atom bombardment , matrix (chemical analysis) , ionization , chemical ionization , electrospray ionization , desorption , moiety , analytical chemistry (journal) , mass spectrum , chromatography , organic chemistry , ion , medicine , alternative medicine , pathology , adsorption
During the control of a multistep organic synthesis on a soluble polymer (PEG) by matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry, a chemical reactivity was encountered when the matrix was acidic, for the samples where the amino moiety of the anchored compounds was protected as a Schiff base. Such imine hydrolysis was proven to be solely mediated by the acidic matrix during analyses since the expected protected structures were detected when the experiments were duplicated with a non‐acidic matrix. Even if MALDI mass spectrometry was found to be more convenient than electrospray ionization mass spectrometry for the monitoring of liquid phase organic syntheses, the chemical reactivity imparted by the use of a matrix must be taken into account to avoid erroneous spectra interpretations. Copyright © 1999 John Wiley & Sons, Ltd.