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Comparison between collision induced dissociation of electrosprayed protonated peptides in the up‐front source region and in a low‐energy collision cell
Author(s) -
van Dongen W. D.,
van Wijk J. I. T.,
Green B. N.,
Heerma W.,
Haverkamp J.
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990915)13:17<1712::aid-rcm703>3.0.co;2-8
Subject(s) - chemistry , collision induced dissociation , collision , dissociation (chemistry) , protonation , tandem mass spectrometry , spectral line , mass spectrometry , quadrupole , tandem , mass spectrum , analytical chemistry (journal) , ion , atomic physics , chromatography , organic chemistry , physics , materials science , computer security , astronomy , computer science , composite material
A systematic comparison between the up‐front Collision induced dissociation (CID) mass spectra and low‐energy CID tandem mass spectra from twenty‐one singly and/or doubly charged peptides has been made. CID spectra of the peptides were recorded at different electrode voltages in the up‐front source region of a single quadrupole instrument and different collision energies in the collision cell of a tandem quadrupole instrument. It was observed that up‐front CID and low‐energy CID yielded comparable product ion spectra from protonated peptides, and that the instrumental settings necessary for obtaining comparable CID mass spectra from the two methods are correlated. Copyright © 1999 John Wiley & Sons, Ltd.