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Energy‐resolved mass spectrometry: a comparison of quadrupole cell and cone‐voltage collision‐induced dissociation
Author(s) -
Harrison Alex G.
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990830)13:16<1663::aid-rcm695>3.0.co;2-t
Subject(s) - chemistry , fragmentation (computing) , mass spectrometry , dissociation (chemistry) , collision induced dissociation , quadrupole , analytical chemistry (journal) , ionization , collision , mass spectrum , atomic physics , electric field , tandem mass spectrometry , ion , chromatography , physics , organic chemistry , computer security , computer science , operating system , quantum mechanics
Collision‐induced dissociation (CID) can be effected in the interface region between atmospheric pressure ionization sources and single quadrupole mass analyzers. By varying the electric field in these cone‐voltage CID experiments energy‐resolved mass spectra can be obtained leading to breakdown graphs exploring the energy evolution of the fragmentation pathways. The breakdown graphs obtained from these cone‐voltage CID studies are very comparable to those obtained by varying the collision energy in the quadrupole collision cell of a BEqQ mass spectrometer. The comparison has been made for the protonated peptides H‐Leu‐Gly‐Gly‐OH, H‐Gly‐Leu‐Gly‐OH, H‐Gly‐Gly‐Leu‐OH and Leu‐enkephalin Copyright © 1999 John Wiley & Sons, Ltd.