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Rapid characterization of urinary metabolites of pibutidine hydrochloride in humans by liquid chromatography/electrospray ionization tandem mass spectrometry
Author(s) -
Kato K.,
Jingu S.,
Ogawa N.,
Higuchi S.
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990815)13:15<1626::aid-rcm689>3.0.co;2-f
Subject(s) - chemistry , chromatography , electrospray ionization , tandem mass spectrometry , mass spectrometry , collision induced dissociation , electrospray , liquid chromatography–mass spectrometry , urine , biochemistry
The metabolic products of pibutidine hydrochloride, a new H 2 ‐receptor antagonist, in human urine after oral administration of 40 mg/man were characterized by high‐performance liquid chromatography/tandem mass spectrometry (LC/MS/MS) with electrospray ionization (ESI). Two‐stage collision‐induced dissociation (CID) experiments, with in‐source CID by increasing the octapole offset voltage and collision‐cell CID, were performed in order to develop a very rapid screening procedure that enhanced selectivity toward pibutidine‐related compounds. It was possible to detect metabolites of pibutidine directly from a crude biological matrix without prior extraction, enabling confirmation of the identity of eight metabolites in urine. In addition, the linear range in ESI for pibutidine‐related compounds was studied to determine the urinary excretion of pibutidine and its metabolites in humans. Copyright © 1999 John Wiley & Sons, Ltd.