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A simple liquid–liquid extraction with hexane for low‐picogram determination of drugs and their metabolites in plasma by high‐performance liquid chromatography with positive ion electrospray tandem mass spectrometry
Author(s) -
Xia YuanQing,
Whigan Daisy B.,
Jemal Mohammed
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990815)13:15<1611::aid-rcm683>3.0.co;2-c
Subject(s) - chemistry , chromatography , electrospray , tandem mass spectrometry , hexane , extraction (chemistry) , liquid chromatography–mass spectrometry , mass spectrometry , electrospray ionization , metabolite , liquid–liquid extraction , high performance liquid chromatography , biochemistry
Four sensitive, specific and accurate methods, based on high‐performance liquid chromatography (HPLC) with positive ion electrospray tandem mass spectrometry (MS/MS) coupled with liquid‐Liquid extraction (LLE), have been developed and validated for the low‐picogram determination of two drug candidates and a metabolite (compounds I–III) in human, monkey and rat plasma. In the LLE procedure, hexane or a mixture of hexane and methyl t ‐butyl ether was used to isolate these compounds from plasma of the different species after basification of each biological sample with sodium carbonate. The reconstituted extracts were then injected into a positive ion electrospray LC/MS/MS system for the quantitative analysis. The lower limit of quantitation of the methods ranged from 20 to 200 pg/mL. The use of hexane for the LLE proved to be simple, rapid and reproducible, and provided very clean extracts with little interference. The inter‐ and intra‐day precision for the four methods was within 9%, and the accuracy was in the range 94–107%. The effect of pH on the isomerization of I ( E ‐isomer) to its Z ‐isomer (II) showed that the rate of isomerization increased with decrease in pH and that there was no isomerization at pH ≥6. Copyright © 1999 John Wiley & Sons, Ltd.