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Matrix‐assisted laser desorption/ionization mass spectrometry in evaluation of protein profiles of infant formulae
Author(s) -
Sabbadin Stefania,
Seraglia Roberta,
Allegri Graziella,
Bertazzo Antonella,
Traldi Pietro
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990730)13:14<1438::aid-rcm663>3.0.co;2-z
Subject(s) - chemistry , mass spectrometry , breast milk , context (archaeology) , infant formula , ionization , matrix (chemical analysis) , characterization (materials science) , laser , matrix assisted laser desorption/ionization , analytical chemistry (journal) , chromatography , desorption , food science , ion , nanotechnology , biochemistry , organic chemistry , optics , paleontology , materials science , adsorption , biology , physics
Breast‐feeding is the nutritional model in humans, and is continued after birth for variable periods. Milk represents an essential source of growth from both nutritional and functional points of view. When nursing is not possible, indicated or sufficient, artificial feeding becomes essential. Substitutes for mother's milk are usually obtained by modifying the composition of cow's milk either qualitatively or quantitatively. The changes usually involve enzymatic and/or thermal treatment, and for this reason a description of the protein profiles of milk formulae is of great interest. After examination of the results obtained by matrix‐assisted laser desorption/ionization (MALDI) in the characterization of protein profiles of cow's milk after different thermal treatments, the application of this analytical technique in the above context appeared interesting. MALDI data for eleven milk formulae, directly acquired from the market, are described and discussed. Results indicate that MALDI mass spectrometry is a particularly powerful method which may be employed either during the production stages of milk formulae or to study the relationships between milk protein content and clinical formula evaluation. Copyright © 1999 John Wiley & Sons, Ltd.