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The effects of sample preparation methods on the variability of the electrospray ionization response for model drug compounds
Author(s) -
Bonfiglio Ryan,
King Richard C.,
Olah Timothy V.,
Merkle Kara
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990630)13:12<1175::aid-rcm639>3.0.co;2-0
Subject(s) - chemistry , chromatography , protein precipitation , analyte , electrospray ionization , electrospray , mass spectrometry , sample preparation , tandem mass spectrometry , phenacetin , extraction (chemistry) , analytical chemistry (journal)
A post‐column infusion system was developed in order to analyze suppression of electrospray ionization (ESI) tandem mass spectrometry response in the presence of endogenous plasma interferences. By enabling direct detection of these interfering components, this experimental system was used to analyze the ability of several common extraction procedures to remove endogenous plasma components that cause changes in the ESI response of model drug substances. Methyl‐ t ‐butyl ether (MTBE) liquid‐liquid, Oasis and Empore solid‐phase, and acetonitrile (ACN) protein precipitation sample preparation methods were tested using the post‐column infusion system. In all cases, ACN protein precipitation samples showed the greatest amount of ESI response suppression while liquid‐liquid extracts demonstrated the least. In addition, the three test compounds, phenacetin, caffeine, and a representative Merck compound, demonstrated that ESI response suppression is compound dependent. Suppression was greatest with caffeine, the most polar analyte, and the smallest for the Merck compound, the least polar analyte. Copyright © 1999 John Wiley & Sons, Ltd.