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Differentiation of epimeric mono‐ and dihydroxypipecolic acids by negative ion sequential mass spectrometry
Author(s) -
Kite Geoffrey C.
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990615)13:11<1063::aid-rcm609>3.0.co;2-f
Subject(s) - chemistry , epimer , mass spectrometry , chromatography , mass spectrum , dissociation (chemistry) , ion , polyatomic ion , chemical ionization , collision induced dissociation , atmospheric pressure chemical ionization , ionization , analytical chemistry (journal) , tandem mass spectrometry , stereochemistry , organic chemistry
Sequential mass spectrometry (MS/MS) using an atmospheric pressure chemical ionization (APCI) source is a useful technique for the identification of the epimeric mono‐ and dihydroxypipecolic acids that occur naturally in plants. In negative ion mode, the collision induced dissociation (CID) spectra of the two 4‐hydroxypipecolic acid epimers, the two 5‐hydroxypipecolic acid epimers and two of the four 4,5‐dihydroxypipecolic acid epimers were all different. In positive ion mode, only two monohydroxypipecolic acids and one dihydroxypipecolic acid could be distinguished from their CID spectra. The two dihydroxypipecolic acid epimers not resolved from negative ion MS/MS could be separated by prior high‐performance liquid chromatography (LC). In a mixture of all eight hydroxypipecolic acids, six could be identified by negative ion LC/MS/MS. The two unresolved dihydroxypipecolic acids co‐eluted and did not produce suitably characteristic fragment ions for single product ion monitoring. Copyright © 1999 John Wiley & Sons, Ltd.