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Rapid typing of Bacillus subtilis strains by their secondary metabolites using matrix‐assisted laser desorption/ionization mass spectrometry of intact cells
Author(s) -
Leenders Frank,
Stein Torsten H.,
Kablitz Bärbel,
Franke Peter,
Vater Joachim
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990530)13:10<943::aid-rcm591>3.0.co;2-0
Subject(s) - chemistry , surfactin , bacillus subtilis , chromatography , mass spectrometry , lipopeptide , matrix assisted laser desorption/ionization , desorption , metabolite , agar , bacteria , biochemistry , organic chemistry , genetics , adsorption , biology
Matrix‐assisted laser desorption/ionization (MALDI) time‐of‐flight mass spectrometry (TOFMS) has been used for rapid and efficient typing of Bacillus subtilis strains according to their secondary metabolite products using whole intact cells as targets. Cells were grown either in liquid media or on agar plates, respectively. Strains were classified by their unique spectra of bioactive peptides in the m/z range from 500 to 5000. The lipopeptide products surfactin, fengycin and the members of the iturin family were structurally characterized in situ by post‐source decay (PSD) analysis. The potential of MALDI‐MS for taxonomic studies, as well as for the investigation of the regulation and compartimentalization of secondary metabolites in the bacterial cell, is demonstrated. These achievements qualify this methodology for rapid screening of microorganisms to exploit bioactive compounds for biotechnological and pharmaceutical applications. Copyright © 1999 John Wiley & Sons, Ltd.