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Determination of major triacylglycerol components of polyunsaturated specialty oils using matrix‐assisted laser desorption/ ionization time‐of‐flight mass spectrometry
Author(s) -
Ayorinde F. O.,
Eribo B. E.,
Balan K. V.,
Johnson J. H.,
Wan L. W.
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990530)13:10<937::aid-rcm590>3.0.co;2-t
Subject(s) - chemistry , mass spectrometry , chromatography , mass spectrum
Five specialty oils; flaxseed, walnut, sesame, grapeseed and hazelnut, have been analyzed by matrix‐assisted laser desorption ionization (MALDI) time‐of‐flight (TOF) mass spectrometry (MS). α‐Cyano‐4‐hydroxycinnamic acid, dissolved in acetonitrile/tetrahydrofuran, was used as the matrix. The MALDI‐TOF mass spectra produced only sodiated triacylyglycerol molecules [M + Na] + . The flaxseed oil contained ten major triacylglycerols (TAGs) which include triolein (OOO), linoleoyldioleoylglycerol (OOL), dilinoleoyloleoylglycerol (OLL), trilinolein (LLL), linolenoyldilinoleoylglycerol (LLLn), dilinolenoyllinoleoylglycerol (LLnLn), trilinolenin (LnLnLn), dilinoleoylpalmitoylglycerol (PLL), linolenoyllinoleoylpalmitoylglycerol (PLLn), and dilinolenoylpalmitoylglycerol (PLnLn). The walnut oil also contained ten major TAGs that were identified as: OOO, OOL, OLL, LLL, LLLn, LLnLn, POO, POL, PLL, and PLLn. Eight major TAGs were identified in the spectrum of sesame oil, including dioleoylstearoylglycerol (SOO), OOO, OOL, OLL, LLL, POO, POL, and PLL. The grapeseed oil was found to contain seven major TAGs: OOO, OOL, OLL, LLL, POO, POL, and PLL. Six major TAGs were identified in the spectrum of hazelnut oil as OOO, OOL, OLL, LLL, POO, and POL. When palmitoyldistearoylglycerol (PSS) was used as an internal mass calibrant with each of the specialty oils, there was a suppression of the sodiated PSS signals, implying that TAGs containing unsaturated fatty acids may have greater affinities for sodium ions than those that contain only saturated acid moieties. The implication of such a trend is that a sample preparation method that does not significantly discriminate against saturated TAGs should be developed for quantitative studies involving mixtures of saturated and unsaturated TAGs. Copyright © 1999 John Wiley & Sons, Ltd.