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In situ determination of organic reaction products by combining thin layer chromatography with surface‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Chen YuChie
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990515)13:9<821::aid-rcm573>3.0.co;2-t
Subject(s) - chemistry , mass spectrometry , chromatography , desorption , analyte , thin layer chromatography , matrix (chemical analysis) , analytical chemistry (journal) , ionization , in situ , matrix assisted laser desorption/ionization , organic chemistry , adsorption , ion
This study presents an in situ determination of organic reaction products on a thin layer chromatography (TLC) plate using surface‐assisted laser desorption/ionization (SALDI) time‐of‐flight mass spectrometry. This technique can rapidly identify products and byproducts on an intact TLC plate. The characteristics of low matrix interference and low suppression effects in SALDI make this technique particularly appropriate for small organic compounds. In addition, the SALDI liquid used in the SALDI matrix system helps to reduce the interaction between analyte and silica gel on the TLC plate, thus avoiding an extra extraction procedure when preparing the sample. Furthermore, preparing the sample by combining TLC with SALDI mass spectrometry on the TLC plate is a relatively easy task. A TLC/SALDI analysis takes less than ten minutes to complete. Copyright © 1999 John Wiley & Sons, Ltd.