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Analysis of metallothioneins by means of capillary electrophoresis coupled to electrospray mass spectrometry with sheathless interfacing
Author(s) -
Guo Xu,
Chan Hing Man,
Guevremont Roger,
Siu K. W. Michael
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990330)13:6<500::aid-rcm515>3.0.co;2-g
Subject(s) - chemistry , electrospray , capillary electrophoresis , metallothionein , electrospray mass spectrometry , mass spectrometry , chromatography , gene isoform , electrophoresis , acetylation , analytical chemistry (journal) , biochemistry , gene
Capillary electrophoresis (CE) coupled to electrospray mass spectrometry via sheathless interfacing has been applied to the analysis of mammalian metallothionein (MT) extracts. In a rabbit‐liver extract, four (MT‐2C, MT‐2A, MT‐2D and MT‐2E) out of six known MT sub‐isoforms were unambiguously identified under three CE‐resolved peaks. A fourth peak was found to contain MT‐1A and/or MT‐2B, whose molecular masses differ by only 1 Da. Traces of non‐N‐acetylated MT‐2D and MT‐2E were observed in a fifth, minor peak. In a rat‐liver extract, both MT‐1 and MT‐2 were resolved and identified. Non‐N‐acetylated MT‐2 was also identified in a resolved, minor peak. Minimum detectable amounts of MTs have been estimated to be approximately 0.6 fmol per sub‐isoform. Copyright © 1999 John Wiley & Sons, Ltd.