Premium
Structural determination of synthetic monoacetyldiglycerides by tandem mass spectrometry of sodium‐adducted molecules
Author(s) -
Kim Young Hwan,
Han SoYeop,
Cho SoHye,
Yoo Jong Shin,
Jhon GilJa
Publication year - 1999
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/(sici)1097-0231(19990330)13:6<481::aid-rcm511>3.0.co;2-k
Subject(s) - chemistry , tandem mass spectrometry , fragmentation (computing) , fast atom bombardment , mass spectrometry , molecule , collision induced dissociation , tandem , ion , sodium , dissociation (chemistry) , stereochemistry , analytical chemistry (journal) , chromatography , organic chemistry , materials science , composite material , computer science , operating system
A variety of synthetic monoacetyldiglycerides (MADGs), which show hematopoietic stem cell stimulating activity, were investigated using high‐energy collision‐induced dissociation (CID) tandem mass spectrometry (MS/MS) coupled with fast‐atom bombardment (FAB). CID of sodium‐adducted molecules, [M + Na] + , generated product ions highly informative on their structure. In particular, two type of ions (named E 2,3 and J) and the relative intensity ratio (G 2 /G 1 ) of two product ions (named G 1 and G 2 ) by the loss of free fatty acids (‐R n ′ COOH) provided definitive information about the position of two long‐chain acyl groups on the glycerol backbone. In addition, charge‐remote fragmentation (CRF) along the long hydrocarbon chains of two fatty acyl groups determined the position of each double bond. Thus, this direct and rapid method can be applied to the structural determination of individual molecular species in a mixture of MADGs isolated from biological origins. Copyright © 1999 John Wiley & Sons, Ltd.